Regulatory

Part:BBa_M36011:Design

Designed by: Brent Townshend   Group: Stanford BIOE44 - S11   (2011-04-26)

Fur-regulated (Fe) Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 159
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 159
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 159
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 159
    Illegal NgoMIV site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on Gunter et al paper which performed deletion analysis of natural S. lividans gene.

Region 312-475 of Sau3AI fragment from pTQ209, referred to as pPS-PCR. In the Gunter paper, this is shown as Figure 2, with the sequence ending just after the SpeI site.

This promoter was tested by Gunter by following it with a Beta-galactosidase coding region and measuring activity for cells grown 48h in iron-rich (+Fe) or iron-limited (-Fe) medium. Activity in S. lividans was 1130 mU/mg for -Fe and 27 mU/mg for +Fe.

Source

1. Gunter K, Toupet C, Schupp T. Characterization of an iron-regulated promoter involved in desferrioxamine B synthesis in Streptomyces pilosus: repressor-binding site and homology to the diphtheria toxin gene promoter. Journal of bacteriology. 1993;175(11):3295-302. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=204726&tool=pmcentrez&rendertype=abstract.

References